The simplest way to subsequently program a dilution series is to highlight a certain number of wells, and then define the desired starting and end concentrations of the compound and decide whether the dilution scheme should be logarithmic or linear. First, parameters such as the plate format (12- to 384-well plates are supported), the assay volume, the maximally tolerated DMSO concentration, and the names and stock concentrations of the compounds to be dispensed are defined.
It is possible to generate custom-made dilution series of DMSO-dissolved compounds rapidly and accurately, with minimized compound consumption and with minimal effort. The remarkably small volume that is transferred with a single shot allows the direct dispensing of stock solutions into assay plates, spanning a wide range of concentrations of the dispensed compound. The device dispenses single droplets of either 20 or 13 picoliters, and larger volumes are digitally built by rapidly shooting multiple droplets into the same well, just like ink-jet printers shoot ink droplets on paper to print letters and words. In this article, we introduce a benchtop instrument, HP D300 Digital Dispenser, available from Tecan, that reliably and rapidly dispenses small volumes of DMSO-dissolved compounds directly into wells of microtiter plates in a contact-free manner directly from stock solutions. The pipetting of serial dilution series is daily business in biomedical laboratories, be it for the determination of the effect of test compounds in biochemical or cellular assays, for finding optimal concentrations of substrates and co-factors in assay development, or for a wide range of enzymological studies, such as active site titrations or determinations of kinetic constants of slow binding and irreversible inhibitors. Use of Digital Dispensing to Facilitate an Accurate, Fast, and Facile Workflow
0 kommentar(er)
